Associate professor
Graduate School of Information Science and Technology, Osaka University

[Google Scholar]

Research Projects


〒565-0871 1-5 Yamada-oka, Suita-shi, Osaka-fu
Information Science and Technology, Osaka University

Tel: 06-6879-4151


2003-2005 PhD at the University of Tokyo, Graduate School of Pharmaceutical Sciences (Supervisor: Professor Kazuhisa Sekimizu)
Microbiology, Biochemistry
2007-2008 Postdoc Researcher at ERATO project of Japan Science and Technology Agency
Construction of cell-like reactors
2008-2009 Specially appointed assistant professor at Osaka University, Graduate School of Information Science and Technology
Evovable artificial cell
2013- present Associate Professor at Osaka University, Graduate School of Information Science and Technology



  1. Sakatani, Y., Yomo, T., Ichihashi, N.*
    Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination.
    Sci Rep 8, 13089 (2018).
  2. Mizuuchi, R. and Ichihashi, N.*
    Sustainable replication and coevolution of cooperative RNAs in an artificial cell-like system.
    Nat Ecol Evol. In press (2018).
  3. Yoshiyama, T., Ichii, T., Yomo, T., Ichihashi, N.*
    Automated in vitro evolution of a translation-coupled RNA replication system in a droplet flow reactor.
    Sci Rep 8, 11867. (2018).
  4. Tsuji, G., Sunami, T., Ichihashi, N.
    Production of giant unilamellar vesicles by the water-in-oil emulsion-transfer method without high internal concentrations of sugars. J Biosci Bioeng. in press (2018)
  5. Furubayashi, T., Ichihashi, N.
    Sustainability of a compartmentalized host-parasite replicator system
    Life, 8(1), 3; doi: 10.3390/life8010003 (2018)
  6. Murase, Y., Nakanishi, H., Tsuji, G., Sunami, S., Ichihashi, N.
    In vitro evolution of unmodified 16S rRNA for simple ribosome reconstitution
    ACS Synthetic Biology, in press (2017)
  7. Tsuji, S., Ichihashi, N.
    Translation activity of chimeric ribosomes composed of Escherichia coli and Bacillus subtilis or Geobacillus stearothermophilus subunits
    Biochem Biophys Rep, 10, 325-328 (2017)
  8. Yumura, M., Yamamoto, N., Yokoyama, K., Mori, H., Yomo, T., Ichihashi, N.
    Combinatorial selection for replicable RNA by Qβ replicase while maintaining encoded gene function
    Plos ONE, 12(3): e0174130 (2017)
  9. Aita, T., Ichihashi, N., Yomo, T.
    Inference of fitness values and putative appearance time points for evolvable self-replicating molecules from time series of occurrence frequencies in an evolution reactor.
    J Theoretical Biol, 401, 38-42 (2016)
  10. Sunami, T., Ichihashi, N., Nishikawa, T., Kazuta, Y., Yomo, T.
    Effect of Liposome Size on Internal RNA Replication Coupled with Replicase Translation.
    ChemBioChem, 17, 1282-1289 (2016)
  11. Mizuuchi, R., Ichihashi, N.*, Yomo, T. (*corresponding)
    Adaptation and diversification of an RNA replication system under initiation- or termination-impaired translational conditions.
    ChemBioChem, 17, 1229-1232 (2016)
  12. Bansho, Y., Furubayashi, T., Ichihashi, N.*, Yomo, T.* (*co-corresponding)
    Host-parasite oscillation dynamics and evolution in a compartmentalized RNA replication system.
  13. Furubayashi T, Nakano T, Eckford A, Okaie Y, Yomo T.
    Packet Fragmentation and Reassembly in Molecular Communication.
    IEEE Transactions on NanoBioscience ,(99),1-5.(2016)
  14. Taro Furubayashi, Tadashi Nakano, Andrew Eckford, Norikazu Ichihashi, Tetsuya Yomo.
    Packet Replication and Noise in Reliable End-to-end Molecular Communication.
    9th EAI International Conference on Bio-inspired Information and Communications Technologies,1-2.(2015)
  15. Taro Furubayashi, Tadashi Nakano, Andrew Eckford, Tetsuya Yomo.
    Reliable End-to-end Molecular Communication with Packet Replication and Retransmission.
    2015 IEEE Global Communications Conference (GLOBECOM),1-6.(2015)
  16. Tomita K, Ichihashi N, Yomo T.
    Replication of partial double-stranded RNAs by Qβ replicase.
    Biochem Biophys Res Commun,467(2),293-296.(2015)
  17. Yoshiyama T, Motohashi C, Ichihashi N, Ichii T, Yomo T.
    Maintenance of a translation-coupled RNA replication in dynamic micro-sized compartments under continuous stirring and flow. Chemical Engineering Journa,283(14015),896-902.(2015)
  18. Ichihashi N, Aita T, Motooka D, Nakamura S, Yomo T.
    Periodic pattern of genetic and fitness diversity during evolution of an artificial cell-like system. Molecular Biology and Evolution,32(12),3205-3214.(2015)
  19. Takako Awai, Norikazu Ichihashi,Tetsuya Yomo.
    Activities of 20 aminoacyl-tRNA synthetases expressed in a reconstituted translation system in Escherichia coli. Biochemistry and Biophysics Reports,3,140-143.(2015)
  20. Usui, K.*, Ichihashi, N.*, Yomo, T. (*equally contributed)
    A design principle for a single-stranded RNA genome that replicates with less double-strand formation.
    Nucleic Acids Research,43(16),8033-8043. (2015)
  21. Sakatani Y, Ichihashi N, Kazuta Y, Yomo T.
    A transcription and translation-coupled DNA replication system using rolling-circle replication.
    Scientific Reports,5,(10404),10.1038/srep10404,1-9.(2015)
  22. Kotaro Nishiyama, Norikazu Ichihashi, Yasuaki Kazuta, Tetsuya Yomo.
    Development of a reporter peptide that catalytically produces a fluorescent signal through α-complementation.
    Protein Science,24,(5),599-603.(2015)
  23. Ichihashi N, Kobori S, Yomo T.
    Simple identification of two causes of noise in an aptazyme system by monitoring cell-free transcription.
    Methods in Enzymology,550,93-107.(2015)
  24. Uno K, Sunami T, Ichihashi N, Kazuta Y, Matsuura T, Yomo T.
    The Evolutionary Enhancement of Genotype-Phenotype Linkages in the Presence of Multiple Copies of Genetic Material.
  25. Ryo Mizuuchi,Norikazu Ichihashi,Kimihito Usui,Yasuaki Kazuta,and Tetsuya Yomo.
    Adaptive Evolution of an Artificial RNA Genome to a Reduced Ribosome .
    ACS Synthetic Biology,4(3),292-298.(2014)
  26. Yasuaki Kazuta,Tomoaki Matsuura,Norikazu Ichihashi,Tetsuya Yomo.
    Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system.
    Journal of Bioscience and Bioengineering,118(5),554-557.(2014)
  27. Kimihito Usui, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Tetsuya Yomo.
    Effects of ribosomes on the kinetics of Qb replication.
    FEBS Letters.,588(1),117-123.(2014)
  28. Takuyo Aita, Norikazu Ichihashia, Tetsuya Yomo.
    Probabilistic model based error correction in a set of various mutant sequences analyzed by next-generation sequencing.
    Computational Biology and Chemistry.,2013(47),221-230. (2013)
  29. Norikazu Ichihashi, Kimihito Usui, Yasuaki Kazuta, Takeshi Sunami, Tomoaki Matsuura, Tetsuya Yomo.
    Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment.
    Nature Communications.,4(2494),1-7. (2013)
  30. Kimihito Usui, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Tetsuya Yomo.
    Kinetic model of double-stranded RNA formation during long RNA replication by Qβ replicase.
    FEBS Letters.,2013(587),2565-2571.(2013)
  31. Shungo Kobori, Norikazu Ichihashi, Yasuaki Kazuta, Tetsuya Yomo.
    A controllable gene expression system in liposomes that includes a positive feedback loop.
    Mol. BioSyst.,9(6),1282-1285.(2013)
  32. Kotaro Nishiyama, Norikazu Ichihashi, Tomoaki Matsuura, Yasuaki Kazuta, Tetsuya Yomo.
    α-Complementation in an artificial genome replication system in liposomes.
    Chembiochem. ,2012(13),2701-2706.(2012)
  33. Takehiro Nishikawa, Takeshi Sunami, TomoakiMatsuura, Tetsuya Yomo.
    Directed Evolution of Proteins through In Vitro Protein Synthesis in Liposomes.
    Journal of Nucleic Acids.,2012,1-11.(2012)
  34. Tomoaki Matsuura, Kazufumi Hosoda, Yasuaki Kazuta, Norikazu Ichihashi, Hiroaki Suzuki, Tetsuya Yomo.
    Effects of Compartment Size on the Kinetics of Intracompartmental Multimeric Protein Synthesis.
    ACS Synth. Biol.,2012(1),1431-1437.(2012)
  35. Shungo Kobori, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Tetsuya Yomo.
    Kinetic analysis of aptazyme-regulated gene expression in a cell-free translation system:modeling of ligand-dependent and -independent expression.
  36. Yohsuke Bansho, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo.(2012) Importance of parasite RNA species repression for prolonged translation-coupled RNA self-replication.
    Chemistry and Biology.,19(4),478-487.
  37. Takehiro Nishikawa, Takeshi Sunami, Tomoaki Matsuura, Norikazu Ichihashi, Tetsuya Yomo.(2012) Construction of Gene Screening System Using Giant Unilamellar Liposomes and Fluorescence Activated Cell Sorter.
    Analytical chemistry 2012.,84,5017-5024.
  38. Tomoaki Matsuura, Kazufumi Hosoda, Norikazu Ichihashi, Yasuaki Kazuta, Tetsuya Yomo.
    Kinetic Analysis of -Galactosidase and -Glucuronidase Tetramerization Coupled with Protein Translation.
    Journal of Biological Chemistry.,286(25),22028-22034.(2011)
  39. Norikazu Ichihashi, Tomoaki Matsuura, Kazufumi Hosoda and Tetsuya Yomo.
    Identification of two forms of Qβ replicase with different thermal stabilities but identical RNA replication activity.
    The Journal of Biological Chemistry.,285(48), 37210-37217.(2010)
  40. Hiroya Urabe, Norikazu Ichihashi, Tomoaki Matsuura, Kazufumi Hosoda, Yasuaki Kazuta, Hiroshi Kita, Tetsuya Yomo.
    Compartmentalization in a water-in-oil emulsion repressed the spontaneous amplification of RNA by Qbeta replicase. Biochemistry, 49(9), 1809-1813.(2010)
  41. Kurokawa, K., Mizumura, H., Takaki, T., Ishii, Y., Ichihashi, N., Bok Luel Lee, Sekimizu, K. Rapid Exchange of Bound ADP on the Staphylococcus aureus Replication Initiation protein DnaA.
    Journal of Biological Chemistry, 284, 34201-10, (2009)
  42. Ichihashi, N., Matsuura, T., Kita, H., Hosoda, K., Sunami, T., Tsukada, K., and Yomo, T.
    Importance of translation-replication balance for efficient replication by the self-encoded replicase
    ChemBioChem, 9 (18),3023-3028. (2008)
  43. Kita, H., Matsuura, T., Sunami, T., Hosoda, K., Ichihashi, N., Tsukada, K., Urabe, I., and Yomo T.
    Replication of genetic information with self-encoded replicase in liposomes. ChemBioChem, 9 (15),2403-2410. (2008)
  44. Hosoda, K., Matsuura, T., Kita, H., Ichihashi, N., Tsukada, K., Urabe, I., and Yomo, T.
    A novel sequence-specific RNA quantification method using nicking endonuclease,dual-labeled fluorescent DNA probe, and conformation-interchangeable oligo-DNA. RNA,14,584-592. (2008)
  45. Hosoda, K., Matsuura, T., Kita, H., Ichihashi, N., Tsukada, K.,and Yomo, T.
    Kinetic analysis of the entire RNA amplification process by Qβreplicase.
    J. Biol. Chem., 282 (21),15516-15527. (2007)
  46. Murai, N., Kurokawa, K., Ichihashi, N., Matsuo, M., Sekimizu, K.
    Isolation of a temperature-sensitive dnaA mutant of Staphylococcus aureus.
    FEMS microbiology letters, 254, 19-26, (2006)
  47. Oku, Y., Kurokawa, K., Ichihashi, N., Sekimizu, K.
    Characterization of the Staphylococcus aureus mprF gene, involved in lysinylation of phosphatidylglycerol
    Microbiology 150, 45-51, (2004)
  48. Ichihashi, N., Kurokawa, K., Matsuo, M., Kaito, C., Sekimizu, K.
    Inhibitory effects of basic or neutral phospholipid on acidic phospholipid-mediated dissociation of adenine nucleotide bound to DnaA protein, the initiator of chromosomal DNA replication.
    Journal of Biological Chemistry, 278, 28778-28786, (2003)

  50. Ichihashi, N., and Yomo, T.
    Constructive approaches for understanding the origin of self-replication and evolution
    Life, 6(3), 26 (2016)
  51. [Japanese only]市橋伯一、四方哲也
    生物工学 2015年 第10号 第93巻 7−10
  52. [Japanese only]市橋伯一
    Journal of Japanese Society for Extremophiles 13, 59-62 (2014)
  53. Ichihashi N, Yomo T
    Positive roles of compartmentalization in internal reactions.
    Curr Opin Chem Biol. 22C 12-17 (2014)
  54. [Japanese only]市橋伯一、四方哲也
    高分子 2014年6月号 63巻 376-378
  55. [Japanese only]角南武志、市橋伯一、松浦友亮、鈴木宏明、四方哲也
    実験医学増刊 2011年29, 152-159,
  56. Ichihashi, N., Matsuura, T., Kita, H., Sunami, T., Suzuki, H., Yomo, T.
    Constructing partial models of cells. Cold Spring Harbor Perspective in Biology 2, a004945, 2010.
  57. [Japanese only]市橋伯一、四方哲也
    科学 80, 740-742, 2010年
  58. [Japanese only]市橋伯一
    生物物理 50, 128-129, 2010年


  1. [Japanese only]市橋伯一
    進化する人工細胞の構築と生命の起源 人工細胞の創製とその応用  植田 充美 (監修), 第3章2節 シーエムシー出版 (2017)
  2. Ichihashi, N., Matsuura, T., Kita, H., Sunami, T., Suzuki, H., Yomo, T.
    Constructive approaches for the origin of life. A captor in “Genesis - in the Beginning: Precursors of Life, Chemical Models and Early Biological Evolution.” J. Seckbach Ed., Springer, 2012
  3. Matsuura, T., Ichihashi, N., Sunami, T., Kita, H., Suzuki, H., Yomo, T.
    Evolvability and Self-Replication of Genetic Information in Liposomes A captor in “The Minimal Cell : The Biophysics of Cell Compartment and the Origin of Cell Functionality” Springer, 2010


  1. [Japanese only]四方哲也、市橋伯一、角南武志、西川雄大
    一枚膜リポソームを用いた酵素進化法の開発 特許第5467320号
  2. [Japanese only]四方哲也、松浦友亮、市橋伯一、占部博也
    エマルジョンを用いたRNAの選択的増幅法 特許第5099455号


  • ビジュアルバイオテクノロジー 9章 エボデボ:進化と発生を分子生物学でひもとく 化学同人 Carolyn A. Dehlinger 著, 福井 希一, 内山 進, 松田 史生 監訳, 2017
  • FUNDING[Japanese only]

    1. 科研費 新領域研究 冥王代生物学(公募班) 代表
      2017年度 − 2018年度
    2. 革新的研究開発推進プログラム(ImPACT)「豊かで安全な社会と新しいバイオものづくりを 実現する人工細胞リアクタ」公募研究 代表
      2016年度 − 2018年度
    3. 科研費 基盤B(一般) 代表
      2015年度 − 2017年度
    4. 科研費 基盤B(特設) 代表
      2015年度 − 2019年度
    5. 科研費 新領域研究 新生鎖の生物学(公募班) 代表
      新生鎖研究のためのリボソームin vitro人為選択技術の開発
      2015年度 − 2016年度
    6. 鷹野学術振興財団 研究助成 代表
      2015年 − 2015年
    7. 科研費 挑戦的萌芽研究 代表
      2013年度 − 2014年度
    8. 科研費 若手研究(A) 代表
      2011年度 − 2013年度
    9. 科研費 若手研究(B) 代表
      2010年度 − 2011年度